๐Ÿงช Research Tool

Optimal Primer Design

์œ ์ „์ž ์ด๋ฆ„์„ ์ž…๋ ฅํ•˜๋ฉด off-target์ด ์ตœ์†Œํ™”๋œ ์ตœ์ ์˜ PCR primer๋ฅผ ์ž๋™์œผ๋กœ ์„ค๊ณ„ํ•ฉ๋‹ˆ๋‹ค. Tm, GC%, secondary structure๋ฅผ ๋ชจ๋‘ ๊ณ ๋ คํ•œ ์ •๋ฐ€ ์„ค๊ณ„ ๋„๊ตฌ์ž…๋‹ˆ๋‹ค.

๐Ÿงฌ

Primer ์„ค๊ณ„ ์ค€๋น„ ์™„๋ฃŒ

์œ ์ „์ž๋ฅผ ์ž…๋ ฅํ•˜๊ณ  ์„ค๊ณ„ ๋ฒ„ํŠผ์„ ํด๋ฆญํ•˜์„ธ์š”.
์ตœ์ ์˜ primer pair๋ฅผ ์ž๋™์œผ๋กœ ์ฐพ์•„๋“œ๋ฆฝ๋‹ˆ๋‹ค.

๐Ÿ“‹ ํƒ€๊ฒŸ ์œ ์ „์ž ์ •๋ณด

๐Ÿ”ฌ ์„ค๊ณ„ ์•Œ๊ณ ๋ฆฌ์ฆ˜

1

์„œ์—ด ์ˆ˜์ง‘

NCBI RefSeq / Ensembl์—์„œ transcript ์„œ์—ด ํš๋“

2

Exon ๋งคํ•‘

Exon-exon junction ์œ„์น˜ ๊ณ„์‚ฐ ๋ฐ gDNA ๊ตฌ๋ถ„

3

ํ›„๋ณด ์ƒ์„ฑ

Sliding window๋กœ ๋ชจ๋“  ๊ฐ€๋Šฅํ•œ primer ํ›„๋ณด ์ƒ์„ฑ

4

์—ด์—ญํ•™ ๊ฒ€์ฆ

Tm, GC%, hairpin, self-dimer ๊ณ„์‚ฐ

5

ํŠน์ด์„ฑ ๊ฒ€์‚ฌ

Off-target ๊ฒฐํ•ฉ ๊ฐ€๋Šฅ์„ฑ ํ‰๊ฐ€

6

์ ์ˆ˜ํ™”

13๊ฐ€์ง€ ๊ธฐ์ค€์œผ๋กœ ์ข…ํ•ฉ ์ ์ˆ˜ ์‚ฐ์ถœ